Titlebook: G Protein-Coupled Receptor Signaling; Methods and Protocol Mario Tiberi Book 2019 Springer Science+Business Media, LLC, part of Springer Na

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https://doi.org/10.1007/978-1-349-00752-3ways, the modulation of this enzyme family by that type of receptor can become an important experimental question. Here, we describe two different methods, an in-gel and a colorimetric PTP assay, to evaluate the modulation of PTP activity after stimulation with GPCR agonists.

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Molecular Dynamic Simulations to Probe Water Permeation Pathways of GPCRsputational tools to investigate molecular behavior of proteins and internal water molecules which are related to the function of proteins; however, the MD simulations of the rhodopsin require several technical setups for accurate calculations. This chapter discusses practical methods for setting up

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A Combined Cell-Free Protein Synthesis and Fluorescence-Based Approach to Investigate GPCR Binding Phis context, we present two methods for the site-specific fluorescent labeling of difficult-to-express membrane proteins in combination with cell-free protein synthesis. The cell-free protein synthesis system is based on Chinese Hamster Ovary Cells (CHO) since this system contains endogenous membran

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