Titlebook: Extracellular RNA; Methods and Protocol Tushar Patel Book 2018 Springer Science+Business Media, LLC, part of Springer Nature 2018 biomarker

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Detection and Analysis of Non-vesicular Extracellular RNA,onditioned media of mammalian cell culture. Focus on this fraction will increase our understanding on extracellular RNA biology, while serving as a source for biomarker discovery complementary to EVs.

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Milk-derived Extracellular Vesicles for Therapeutic Delivery of Small Interfering RNAs,s with RNA is possible with the use of chemical transfection reagents. This method describes the use of milk-derived EV for delivery of small interfering RNA. These EVs were shown to be taken up by hepatocellular carcinoma cells in vitro, with a reduction in the expression of target gene.

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Unsaturated Soil Hydraulic Properties,ll culture supernatants have been previously used by investigators. Here, we describe several standardized protocols for the isolation of RNAs from cell culture supernatants that utilize commercially available kits and reagents.

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Differential Equations with Random Delay,es of body fluids which contain protein, mRNA, and noncoding RNA. Extracellular RNA isolated from bile could be a useful tool for analyzing biliary tract diseases or cancer. Herein, we describe protocols based on modifications of commercially available kits for the collection, processing, and isolation of extracellular RNA from bile.

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Tushar PatelIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts

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