Titlebook: Expressed Sequence Tags (ESTs); Generation and Analy John Parkinson Book 2009 Humana Press 2009 Alternative transcripts.Bur.Expression prof

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Expressed Sequence Tags (ESTs)978-1-60327-136-3Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Biographieforschung am bewegten Subjekt, serial subtraction of normalized libraries to maintain the discovery rate. Here we describe in detail, protocols for normalization and subtraction of cDNA libraries followed by an example using the toxic dinoflagellate ..

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Construction of cDNA Libraries: Focus on Protists and Fungi,y are nonaxenic and/or difficult to cultivate. In this chapter, we describe protocols that have been applied successfully to construct and normalize a variety of cDNA libraries from many different species of free-living protists and fungi, and that require only small quantities of cell material.

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Generation of Full-Length cDNA Libraries: Focus on Plants,od using the trehalose-thermostabilized reverse transcriptase has been developed and has become an efficient method for construction of high-content full-length cDNA libraries. We have constructed full-length cDNA libraries from various plants and animals using this method. The protocol of the method is described in this chapter.

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