Titlebook: Experiments in Molecular Biology; Robert J. Slater Book 1986 Springer Science+Business Media LLC 1986 biology.Colon.DNA.enzyme.enzymes.Esc

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https://doi.org/10.1007/978-3-319-55595-9ired gene. Human chromosomal genes are most unlikely to be expressed in bacteria, and even less likely to give the colony a recognizable new phenotype. So how is it possible to select the one required clone from perhaps 100,000 others? By directly probing for the required DNA segment, colony hybridization provides a solution to this problem.

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Restriction Endonuclease Digestion and Agarose Gel Electrophoresis of DNA,ll molecules of the genomic DNA must be cut identically, so that each gives the same set of restriction fragments. With luck, one of these fragments will contain the desired gene, and further restriction of the isolated fragment may allow recovery of a smaller fragment containing little more than the gene.

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Protein (Western) Blotting with Immunodetection,us to the method used to transfer DNA from agarose gels (the Southern Blot, . Chapter 5) and has been given the name “Western” blotting (.–.). There are a number of advantages to working with a protein blot rather than the original gel. These include:

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DNA Isolation and Transformation in ,,s of genetic engineering in theory and would like to supplement their knowledge with practical experience. Many DNA manipulations, however, make use of equipment that is unsuitable for introductory classes with possibly large numbers of students, and require more time than that available in already

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