Titlebook: Experimental Protocols in Biotechnology; Neelima Gupta,Varsha Gupta Book 2020 Springer Science+Business Media, LLC, part of Springer Natur

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,Determination of Michaelis–Menten Enzyme Kinetics Parameters of Alkaline Phosphatase in Clinical Sahus have an important role in almost every cellular process. They play an indispensable role in metabolism, signal transduction cascade, and gene expression which makes them an important target for drug development, bioprocess industry, food industry, and eco-friendly green chemistries. They are eme

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A Novel Technique for the Detection of LncRNAs on Tissue Sections,t lncRNA accounts for most of the noncoding transcriptome in humans, as tens of thousands of lncRNA transcripts have been identified. However, only?a few lncRNAs have been characterized in detail to date. It is known that they act as important regulators of gene expression and are thought to have mu

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1949-2448 ides the latest evidence-based approaches to the diagnosis aThis manual provides insights into the theory and practical aspects of several biotechnological and biochemical techniques for plants, protozoa, nematodes, insects and fishes, as well as human samples. The book also covers bioinformatics to

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John T. Sample,Kevin Shaw,Mahdi Abdelguerfifew lncRNAs have been characterized in detail to date. It is known that they act as important regulators of gene expression and are thought to have multiple functions in cellular and developmental processes. In this chapter we will discuss about the technique for detecting lncRNA from tissue section.

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Jennifer Tsui,Michelle Doose,Kevin A. Henryg of embryos for both fixed and live imaging by confocal microscopy. This collection of protocols allows for analysis of localization of a wide range of proteins including those involved in intracellular transport, cell polarity, cell adhesion, and so on, during early developmental stages of zebrafish embryos and larvae.

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CMV Promoter-Driven Expression and Visualization of Tagged Proteins in Live and Fixed Zebrafish Embg of embryos for both fixed and live imaging by confocal microscopy. This collection of protocols allows for analysis of localization of a wide range of proteins including those involved in intracellular transport, cell polarity, cell adhesion, and so on, during early developmental stages of zebrafish embryos and larvae.

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Bacteriophage Control for , BiofilmFormation and Eradication,l compounds. Bacteriophages are emerging as effective and safe antibacterial alternative strategies in an era of rapidly emerging antibiotic resistance. Basic biofilm assay protocols that can be performed in any microbiology laboratory are reported along with the use of bacteriophages as biocontrol agents.