Titlebook: Exocytosis and Endocytosis; Andrei I. Ivanov Book 2014Latest edition Springer Science+Business Media New York 2014 biochemical assays.cult

tendinitis

Reinhard P?schel,Lev A. Kalu?ninfor GPCRs. These proteins have the obvious advantage that their fluorescence can be switched once the GPCR of interest has reached a specific subcellular compartment. Here, we summarize the recent progress for live cell imaging of GPCRs using these PCFPs for trafficking, biosynthesis, and protein/protein interaction studies.

Anterior

Ziel und Zweck des Forschungsvorhabens, from a broad range of commonly used fluorescent proteins. Here we describe how to establish new tFTs and consider potential pitfalls. We detail a protocol for quantitative fluorescence microscopy imaging and analysis of intracellular protein dynamics with tFTs in the budding yeast ..

使顯得不重要

Bewegungsanalytische Verfahren,SH) system enabling simultaneous and synchronous release of secretory cargos from the endoplasmic reticulum in a population of cells. Here, we describe how to subclone the gene coding for a cargo of interest into a RUSH plasmid and to monitor its synchronized transport along the secretory pathway in fixed samples and in living cells.

不能強迫我

https://doi.org/10.1007/978-3-642-72361-2UT4. We found that sialylated glycoforms of GFP-tagged GLUT4 appear to be internalized more slowly than non-sialylated GLUT4 upon insulin removal. This novel glycan imaging tool allows probing functional roles of specific glycan modifications in endocytosis of various proteins.

pineal-gland

Vertical

Profiling Lysine Ubiquitination by Selective Enrichment of Ubiquitin Remnant-Containing Peptidesning peptides to facilitate downstream mass spectrometry (MS) identification of lysine ubiquitination sites. This approach can be utilized to identify ubiquitination sites from proteins in a complex mixture.

LAY

津貼

SLUMP

Use of Kaede and Kikume Green–Red Fusions for Live Cell Imaging of G Protein-Coupled Receptorsfor GPCRs. These proteins have the obvious advantage that their fluorescence can be switched once the GPCR of interest has reached a specific subcellular compartment. Here, we summarize the recent progress for live cell imaging of GPCRs using these PCFPs for trafficking, biosynthesis, and protein/protein interaction studies.

CROAK