Titlebook: Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation; Narendra Wajapeyee,Romi Gupta Book 2017 Springer Science+B

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Establishment of Time- and Cell-Specific RNAi in ,,cts of genes on various life phenomena. In particular, RNAi is a powerful tool that enables time- or cell-specific knockdown via heat shock-inducible RNAi or cell-specific RNAi. However, the conventional RNAi methods are insufficient for investigating pleiotropic genes with various sites of action a

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Cell-Penetrating Peptide-Mediated Delivery of Cas9 Protein and Guide RNA for Genome Editing,nsists of two components: the Cas9 protein and a guide RNA. To date, delivery of these two components has been achieved using either plasmid or viral vectors or direct delivery of protein and RNA. Plasmid- and virus-free direct delivery of Cas9 protein and guide RNA has several advantages over the c

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Servile

eIF3 Regulation of Protein Synthesis, Tumorigenesis, and Therapeutic Response,ost complex initiation factor consisting of 13 putative subunits. A growing number of studies suggest that eIF3 and its subunits may represent a new group of proto-oncogenes and associates with prognosis. They regulate translation of a subset of mRNAs involved in many cellular processes including pr

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High-Resolution Gene Expression Profiling of RNA Synthesis, Processing, and Decay by Metabolic Laben, transport), and degradation. Profiling these changes provides valuable information on the regulation of gene expression. Total cellular RNA is a poor template for revealing short-term changes in gene expression, alterations in RNA decay rates, and the kinetics of RNA processing as well as the dif

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Profiling Changes in Histone Post-translational Modifications by Top-Down Mass Spectrometry,odifications (i.e., histone code). In this protocol, we describe a top-down workflow that employs liquid chromatography (LC) coupled to mass spectrometry (MS), for fast global profiling of changes in histone proteoforms, and apply LCMS top-down approach for comparative analysis of a wild-type and a

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,Optimizing In Vitro Pre-mRNA 3′ Cleavage Efficiency: Reconstitution from Anion-Exchange Separated HNA 3′ cleavage reaction, which defines the downstream end of the 3′ untranslated region and, in nearly all mRNA, prepares the message for addition of the poly(A) tail. The in vitro reconstitution of 3′ cleavage provides an experimental means to investigate the roles of the various multi-subunit clea

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Unbiased Interrogation of 3D Genome Topology Using Chromosome Conformation Capture Coupled to High-somes are folded in three-dimensional (3D) space. 3C and its derivatives have contributed tremendously to the now widely accepted view that genome topology plays an important role in many major cellular processes, at a chromosome-wide scale, but certainly also at the level of individual genetic loci

PET-scan

Using an Inducible CRISPR-dCas9-KRAB Effector System to Dissect Transcriptional Regulation in Humanlatory networks in pluripotency and potentially in differentiation intermediates of all three germ layers makes this a valuable tool for the stem cell community. Catalytically inactive Cas9 fused to transcriptional/chromatin effector domains allows for silencing or activation of a genomic region of