Titlebook: Lipidomics; Methods and Protocol Sanjoy K. Bhattacharya Book 2023Latest edition The Editor(s) (if applicable) and The Author(s), under excl

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Isolation of Mitochondrial Lipids and Mass Spectrometric Analysis,lation and pathway signaling. The isolation and study of these lipids can provide unparalleled information about the mechanisms behind these cellular processes. In this chapter, we describe a protocol to isolate mitochondrial lipids from homogenized murine optic nerves. The lipid extraction was perf

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Multi-macromolecular Extraction from Endosymbiotic Anthozoans,iodiniaceae comprise the functional basis of all coral reef ecosystems. Given the existential threats of global climate change toward these thermo-sensitive entities, there is an urgent need to better understand the physiological implications of changes in the abiotic milieu of scleractinian corals

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,High-Resolution Liquid Chromatography–Mass Spectrometry for Lipidomics,rformance liquid chromatography (UHPLC) allows for robust separations of complex mixtures, while high-resolution mass spectrometry (HRMS) identifies compounds with efficiency and accuracy (Zullig T and Kofeler HC, Mass Spectrom Rev 40:162–176, 2021). The high specificity and sensitivity of mass spec

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Profiling the Mammalian Lipidome by Quantitative Shotgun Lipidomics,isms. The importance of the lipidome is demonstrated by a number of biological studies on dysregulation of lipid metabolism in human diseases such as cancer, diabetes, and neurodegenerative diseases. Exploring changes and regulations in the huge networks of lipids and their metabolic pathways requir

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