Titlebook: Essentials of Single-Cell Analysis; Concepts, Applicatio Fan-Gang Tseng,Tuhin Subhra Santra Book 2016 Springer-Verlag Berlin Heidelberg 201

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https://doi.org/10.1007/978-3-319-75355-3ical parameters have been used to build models. We describe a motion model based on parameters from dynamic transport, including an anterograde state, a retrograde state, and a pausing state. The residence time between two transition points shows the ability of a motion mode to maintain its state an

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Systems Biology in Single Cells,oming a goal of many research groups. In this chapter we will describe some of the technologies that enable the isolation of individual cells in a form that accommodates systomics studies, the biological methods that are then deployed on such isolated cells to generate system-level information, and

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Electroporation for Single-Cell Analysis, compared to BEP. Single cell analysis using electroporation technique can be performed with microfabricated electrode arrays, carbon fiber microelectrodes, micropipettes or electrolyte-filled capillaries-based devices. Recently developed nanofabricated electrodes can realize localized single cell e

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Microinjection for Single-Cell Analysis,cargo delivery. After introduction of this technique in the early 1900s, numerous modifications were made to improve its efficiency and it was applied to a wide variety of fields from scientific research to clinical therapy. This chapter is intended to provide a basic knowledge of the microinjection

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Optoelectrokinetic Manipulation for Cell Analysis,gets. Unlike some well-adopted techniques, such as dielectrophoresis (DEP), REP and OETs feature high flexibility in manipulation. Their repertoire of manipulation includes, but not limited to, micro/nano concentration, sorting, translation, single particle trapping, and patterning. By combining wit

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Continuous Micro-/Nanofluidic Devices for Single-Cell Analysis,ological and medical assays. These manipulation techniques are frequently combined with other stimulating and sensing techniques for the observation and characterization of single cells, which are described in the second part of the chapter. Major approaches to probe either intact or lysed single ce

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Cytometry of Single-Cells for Biology and Biomedicine,r technologies such as PCR can be added to these multifaceted microfluidic structures. The microfluidic devices can be built to provide low-cost, point-of-care medicine. Making multiple correlated measurements per cell is extremely powerful leading to very large amounts of data of high dimensionalit

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Applications of Single Cell Sequencing in Cancer, possible by minute DNA amplification and nucleotide level resolution made possible by next-generation sequencing, SCS technologies are becoming robust approaches for the study of cancer genomics and transcriptomics, and the cancer–normal cell interactions in the microenvironment. SCS analyses of ca