Titlebook: Epitope Mapping Protocols; Johan Rockberg,Johan Nilvebrant Book 2018Latest edition Springer Science+Business Media, LLC, part of Springer

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Epitope Mapping of Antibodies Using Bacterial Cell Surface Display of Gene Fragment Librariesrmined by DNA sequencing of the sorted antibody-binding cells followed by sequence alignment back to the antigen sequence. The method described here has been useful for the mapping of both monoclonal and polyclonal antibodies with varying sizes of epitopes.

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Mapping Antibody Epitopes by Solution NMR Spectroscopy: Practical Considerationsdy-antigen interactions, and thus to epitope identification. Here, we describe the use of NMR for residue level characterization of protein epitopes, focusing on experimental protocols and practical considerations, highlighting advantages and drawbacks of the approach.

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Introduction

Immunity in genetically obese rodents,btain this information from a diverse range of interactions as described in these protocols, which detail how to grow or capture cells on a surface, how to stabilize and visualize the cells on the surface, and how to set up assays to measure detailed interaction kinetics directly on the cell surface.

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Stephen G. Dennis,Ronald Melzacktocol describes epitope binning of seven human monoclonal antibodies to a monovalent antigen in two orthogonal orientations; a classical sandwich and an in-tandem orientation using an eight-channel Octet system.