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Titlebook: Epigenomics; Methods and Protocol Izuho Hatada,Takuro Horii Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive lice

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發(fā)表于 2025-3-21 17:01:20 | 只看該作者 |倒序瀏覽 |閱讀模式
書目名稱Epigenomics
副標題Methods and Protocol
編輯Izuho Hatada,Takuro Horii
視頻videohttp://file.papertrans.cn/314/313259/313259.mp4
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Epigenomics; Methods and Protocol Izuho Hatada,Takuro Horii Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive lice
描述This detailed volume provides a comprehensive collection of protocols for epigenomic research, powering our ability to analyze epigenetic modifications across the entire genome. Beginning with methods used to investigate epigenomic modifications such as DNA methylation, histone modifications, and chromatin structure, the book continues with methods for manipulating the epigenome, including platforms for epigenome editing, inducible systems for epigenome editing, and epigenetically modified animals. Written for the highly successful .Methods in Molecular Biology. series, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.?.Authoritative and practical, .Epigenomics: Methods and Protocols. serves as an ideal resource for researchers looking to further expand the utility and scope of epigenomics research..
出版日期Book 2023
關鍵詞Epigenetic modifications; CRISPR; dCas9; Next Generation Sequencing (NGS); Epigenome editing
版次1
doihttps://doi.org/10.1007/978-1-0716-2724-2
isbn_softcover978-1-0716-2726-6
isbn_ebook978-1-0716-2724-2Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
The information of publication is updating

書目名稱Epigenomics影響因子(影響力)




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書目名稱Epigenomics網絡公開度




書目名稱Epigenomics網絡公開度學科排名




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書目名稱Epigenomics被引頻次學科排名




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書目名稱Epigenomics年度引用學科排名




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發(fā)表于 2025-3-21 20:30:22 | 只看該作者
https://doi.org/10.1007/3-540-11571-4igenome states enables a detailed analysis of the impact of epigenome status on transcriptional dynamics. In this chapter, we describe a method for quantifying distances between . gene and clusters of cofactor BRD4 using the STREAMING-tag system in mouse embryonic stem cells.
板凳
發(fā)表于 2025-3-22 02:01:36 | 只看該作者
https://doi.org/10.1007/978-3-319-08897-6ust activation of target genes even when strongly silenced. In this chapter, we describe our protocol to design, construct, and validate the TREE-mediated target gene activation in cultured human cells.
地板
發(fā)表于 2025-3-22 06:04:23 | 只看該作者
Book 2023s across the entire genome. Beginning with methods used to investigate epigenomic modifications such as DNA methylation, histone modifications, and chromatin structure, the book continues with methods for manipulating the epigenome, including platforms for epigenome editing, inducible systems for ep
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https://doi.org/10.1007/978-1-4615-6424-9 Our protocols typically require nine cycles of polymerase chain reaction (PCR) amplification to obtain a sufficient amount of library for sequencing when 100?ng of genomic DNA is used as a starting material; moreover, it takes 3?days to complete library preparation and quality control procedures for up to eight samples.
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An Authentic Account of Adam Smithilized embryos, followed by sonication. The nucleoplasmin treatment dramatically increased the efficiency of sperm chromatin solubilization, while a?relatively large amount of recombinant nucleoplasmin was required. Here, we describe an improvement of nucleoplasmin method with a less amount of recombinant protein and a shorter reaction time.
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