Titlebook: Epiblast Stem Cells; Methods and Protocol Pierre Osteil Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license

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Generation of Epiblast-Like CellsESCs in EpiSCs medium, containing bFGF and Activin. Here we report the detailed protocol to generate and characterize the epiblast-like cells (EpiLCs), which correspond to a primed intermediate state between na?ve ESCs and EpiSCs.

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G. Kallos,V. Kotroni,K. Lagouvardos the endoderm precursors to interact with the Matrigel mimicking the laminin-rich basement membrane underlying the egg cylinder epiblast in embryos, and let the precursors migrate into the Matrigel-filled external zone and develop into endodermal epithelial tissues.

只看該作者 · 發(fā)表于 2025-3-28 03:25:25

Definitive Endoderm from EpiSC Aggregates in Matrigel the endoderm precursors to interact with the Matrigel mimicking the laminin-rich basement membrane underlying the egg cylinder epiblast in embryos, and let the precursors migrate into the Matrigel-filled external zone and develop into endodermal epithelial tissues.

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