Titlebook: Eosinophils; Methods and Protocol Garry M. Walsh Book 2021Latest edition Springer Science+Business Media, LLC, part of Springer Nature 2021

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Functionally Active Eosinophil Purification from Peripheral Blood,ometry analysis to evaluate the purity and activation state of isolated cells. In addition, we highlight an in vitro degranulation assay that may be used to verify the intact functionality of the isolated eosinophils.

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Update on Eosinophil Interaction with Mast Cells: The Allergic Effector Unit,ators such as various specific granular mediators, arachidonic acid metabolites, cytokines, and chemokines?[., .]. The allergic effector unit can be studied in vitro in a customized co-culture system using mast cells and eosinophils derived from either mouse or human sources.

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Measurement of Eosinophil Kinetics In Vivo,diolabeled eosinophils coupled to single-photon emission computed tomography (SPECT) to quantify eosinophil uptake in the lungs of healthy volunteers, patients with asthma, and patients with focal eosinophilic inflammation.

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Detection of Eosinophil Extracellular DNA Traps,ls for a semiquantitative and a qualitative analysis of extracellular DNA traps released by human eosinophils, based on fluorimetry and fluorescence microscopy, respectively. These methods can also be applied to detect the DNA trap release by other leukocytes such as neutrophils and even other cell types.

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A History of Aid Effectiveness,dentification and isolation of tissue eosinophils from the small intestine of mice. This protocol is also amenable to the isolation and characterization of colonic eosinophils, and of intestinal eosinophils from human resected tissues.