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Titlebook: Enzymes of Molecular Biology; Michael M. Burrell Book 1993 Humana Press 1993

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書目名稱Enzymes of Molecular Biology
編輯Michael M. Burrell
視頻videohttp://file.papertrans.cn/314/313121/313121.mp4
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Enzymes of Molecular Biology;  Michael M. Burrell Book 1993 Humana Press 1993
描述The scientist‘ s understanding of the cell at the molecular level has advanced rapidly over the last twenty years. This improved understa- ing has led to the development of many new laboratory methods that increasingly allow old problems to be tackled in new ways. Thus the modern scientist cannot specialize in just one field of knowledge, but must be aware of many disciplines. To aid the process of investigation, the Methods Molecular Biology series has brought together many protocols and has highlighted the useful variations and the pitfalls of the different methods. However, protocols frequently cannot be simply taken from the shelf. Thus the starting sample for a chosen protocol may be unavailable in the correct state or form, or the products of the procedure require a different sort of processing. Therefore the scientist needs more detailed information on the nature and requirements of the enzymes being used. This information, though usually available in the literature, is often widely dispersed and frequently occurs in older volumes of journals; not everyone has comprehensive library facilities available. Also many scientists searching out such information are not trained enzy
出版日期Book 1993
版次1
doihttps://doi.org/10.1385/0896032345
isbn_softcover978-0-89603-234-7
isbn_ebook978-1-59259-503-7Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 1993
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1064-3745 older volumes of journals; not everyone has comprehensive library facilities available. Also many scientists searching out such information are not trained enzy978-0-89603-234-7978-1-59259-503-7Series ISSN 1064-3745 Series E-ISSN 1940-6029
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Aminopeptidases,peptides and proteins are genuine posttranslational modifications, or were introduced by cyclization of N-terminal glutamine during purification) or during sequence determination when glutamine was the newly liberated N-terminal amino acid. This cyclized derivative does not have a free amino group,
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https://doi.org/10.1007/978-3-662-60378-9ial can be gaged however by considering that several enzymes implicated in DNA replication, recombination, and repair have integral exo- or endodeoxy-ribonuclease activity. For example, the 5′-3′ and 3′–5′ exonuclease activity of DNA polymerases and the endo-DNase activity of topoisomerases (e.g., .
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Vorstellung der Fallstudie ?KnowBeer“on, parameters for carrying out first-strand cDNA synthesis from poly(A). mRNA populations with AMV RT have been optimized thoroughly (.); standard optima exist for pH, nucleotide concentration, and monovalent and divalent cation concentration; also essential stimulatory additives (such as sodium py
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