Titlebook: Enzyme-Mediated Ligation Methods; Timo Nuijens,Marcel Schmidt Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019

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值得尊敬

Is Caste Already Part of UK Equality Law?,in imaging and diagnostics. Here we describe the modification of proteins using TTL in detail via a one-step as well as two-step procedure and highlight its practicability for applications in imaging, diagnostics, and cell biology.

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Butelase 1-Mediated Ligation of Peptides and Proteins,abeling, preparation of thioesters, and bioconjugation of dendrimers. Additionally, we provide an example using butelase 1 for protein cyclization in combination with genetic code expansion in order to incorporate unnatural building blocks.

Excise

SpyLigase-Catalyzed Modification of Antibodies,rom . cells, chemical solid-phase synthesis of the KTag peptide and its coupling to reporter molecules and cytotoxins as well as the transient expression from mammalian cells to produce Spy-tagged antibodies. Furthermore, we describe the purification and analytics of the formed conjugates.

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Site-Specific Antibody Labeling Using Phosphopantetheinyl Transferase-Catalyzed Ligation, handle that is then used for drug molecule attachment in a second step. The aim of this chapter is to outline detailed protocols for both labeling procedures, as well as to provide guidance on enzyme and substrate preparation.

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重力

Book 2019to view enzymes as a powerful tool in both academic and industrial research. Chapters in this book cover topics such as sortase A-mediated generation of site-specifically conjugated antibody-drug conjugates; omniligase-catalyzed inter- and intramolecular ligation; ligation catalyzed by microbial tra

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1064-3745 ation advice from the experts.This volume discusses different enzyme-catalyzed ligation methodologies for a variety of different chemical transformations. This book wants readers to view enzymes as a powerful tool in both academic and industrial research. Chapters in this book cover topics such as s

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After-Sales-Feedback mit Kundenkonferenzenaction conditions. In this chapter, we describe proximity-based sortase-mediated ligation (PBSL), which uses the SpyTag–SpyCatcher peptide–protein pair to link SrtA to target proteins and dramatically improves reaction rate and ligation efficiency.

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https://doi.org/10.1007/978-981-10-7092-1 .terminal NMT recognition sequence are covalently modified with an azido fatty acid. Subsequent strain-promoted azide–alkyne cycloaddition allows for conjugation to cell-permeant fluorophores and imaging by fluorescence microscopy. Here we describe sample preparation and labeling protocols for imaging bacterial proteins in fixed and live cells.