Titlebook: Enzyme-Mediated Immunoassay; T. T. Ngo,H. M. Lenhoff Book 1985 Plenum Press, New York 1985 Pet.Plasma.Vine.antibody.biology.cell.cell biol

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https://doi.org/10.1007/978-3-662-62325-1label a ligand analyte (L). Such stable covalent enzyme modulator-ligand conjugates (M-L) is capable of modulating the activity of an indicator enzyme by either causing a significant inhibition of the enzyme activity or a dramatic activation.

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https://doi.org/10.1057/9780230355316lity of a stable covalent ligand-prosthetic group conjugate to activate an apoenzyme which by itself is enzymatically inactive; and (2) the ability of anti-ligand antibody to bind ligand-prosthetic group conjugate such that the bound ligand-prosthetic group is incapable of activating the apoenzyme.

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Prosthetic Group Labeled Enzyme Immunoassaylity of a stable covalent ligand-prosthetic group conjugate to activate an apoenzyme which by itself is enzymatically inactive; and (2) the ability of anti-ligand antibody to bind ligand-prosthetic group conjugate such that the bound ligand-prosthetic group is incapable of activating the apoenzyme.

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Nonseparation Enzyme Immunoassays for Macromoleculesdase together with a macromolecular substrate (Gibbons et al., 1980). The second is based on the phenomenon of “enzyme channeling” (Litman et al., 1980) involving two or more enzymes. This chapter will discuss the principles, characteristics, performance and clinical utility of both types of assay.

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978-1-4684-5014-9Plenum Press, New York 1985