Titlebook: Enzyme Technology; III. Rotenburg Ferme Robert MacIntyre Lafferty Conference proceedings 1983 Springer-Verlag, Berlin, Heidelberg 1983 DNA.

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978-3-540-12479-5Springer-Verlag, Berlin, Heidelberg 1983

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Unusual Catalytic Properties of Usual Enzymesher, sometimes quite different processes. The latter, although unimportant for the enzyme-producing organism, can be very valuable for biotechnological applications. The considered examples of “unnatural” catalytic activities of common enzymes, taken from the author’s recent studies include: (I) red

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Purification and Characterization of Membrane-Bound Aldehyde Dehydrogenase from , Grown on Long-Chai This enzyme is induced by exogenous alkanes, alcohols and aldehydes and repressed by intermediates of central metabolic pathways. It was shown ultracytochemically that it is located exclusively in the membranes which envelop intracellular hydrocarbon inclusions. The enzymic activity depends on card

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Application of the Recombinant DNA Technique to Enzyme Technologyveloped for this purpose, including plasmids and phage which allow molecular cloning of DNA in a variety of industrial microorganisms. In a few cases these systems have already been successfully used for enzyme production. Problems which arise during such experiments include non-expression of hetero

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Disintegration of Microorganisms in a 20 1 Industrial Bead Millymes and proteins from suspensions of several yeast and bacterial species was followed during the disruption procedure. Certain operating variables for the disintegration were investigated, in particular the effect of agitator speed, the flow rate, and the bead diameter. The heat production and the