Titlebook: Enzyme Stabilization and Immobilization; Methods and Protocol Shelley D. Minteer Book 2017Latest edition Springer Science+Business Media Ne

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https://doi.org/10.1007/978-3-030-18807-8e oxidase, and a more direct determination of glucose. This chapter describes the methods used to use CNTs in a layer-by-layer structure along with glucose oxidase to produce an enzymatically modified electrode with high turnover rates, increased stability and shelf-life.

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Lipase Activation and Stabilization in Room-Temperature Ionic Liquids,he approach entails the use of salt hydrates to control the level of hydration of the immobilized enzyme, which is critical to the activation of enzymes in nonaqueous media. Although lipase is used as a model enzyme, this approach may be effective in activating and stabilizing virtually any enzyme in RTILs.

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Enzyme Stabilization via Bio-Templated Silicification Reactions,ds described here provide a simple and effective approach for molecular biologists, biochemists and bioengineers to create stable, solid phase biocatalysts that may be integrated within sensors, synthetic processes, reactive barriers, energy conversion, and other biotechnology concepts.

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Layer-by-Layer Assembly of Glucose Oxidase on Carbon Nanotube Modified Electrodes,e oxidase, and a more direct determination of glucose. This chapter describes the methods used to use CNTs in a layer-by-layer structure along with glucose oxidase to produce an enzymatically modified electrode with high turnover rates, increased stability and shelf-life.

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Introduction to the Field of Enzyme Immobilization and Stabilization,i.e., high temperatures), in high substrate concentration or high ionic strength, and in nontraditional solvent systems. This chapter introduces the topic enzyme stabilization and the methods used for enzyme stabilization including enzyme immobilization.

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Protein-Coated Microcrystals, Combi-Protein-Coated Microcrystals, and Cross-Linked Protein-Coated Mion of coffee oil present in spent coffee grounds to biodiesel is described. Cross-linked protein-coated microcrystals (CL-PCMC) in some cases can give better results than PCMC. Protocols for the CLPCMC of Subtilisin Carlsberg and . lipase B (CAL B) are described. A discussion of their applications is also provided.

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FAD-Dependent Glucose Dehydrogenase Immobilization and Mediation Within a Naphthoquinone Redox Polynctionalized redox polymer (NQ-LPEI) that is used to immobilize and electronically communicate with flavin adenine dinucleotide-dependent glucose dehydrogenase (FAD-GDH), yielding an enzymatic bioanode that is able to deliver large catalytic current densities for glucose oxidation at a relatively low associated potential.