Titlebook: Enzyme Immunoassays; From Concept to Prod S. S. Deshpande Book 1996 Chapman & Hall 1996 Antigen.biochemistry.chemistry.classification.devel

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Conjugation Techniquesreagents such as enzyme-antigen or enzyme-antibody conjugates that ultimately determine the sensitivity of the product, is vital to the success of an immunoassay product development process. Conjugation or coupling reactions must preserve the immunogenicity of the hapten, i.e., its antigenic determi

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Antibody Productiondeveloping sensitive, precise, and specific immunoassay products for use in the diagnostics industry. Although the primary objectives of this phase of immunoassay product development appear to be quite simple, i.e., to elicit an immune response, and to select and purify antibodies that have the nece

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Separation and Solid-Phase Systemsibody (or the ligand) must be determined. Since the reaction between the ligand and its specific antibody does not produce a precipitate, it is necessary to effect a physical separation of the two forms before either or both forms can be quantitated. By definition, in all heterogeneous EIAs, a physi

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Immunoassay Classification and Commercial Technologiesal techniques. Although the term “immunoassay” generally refers to a quantitative method, in a much broader sense it also includes characterizing methods for analyzing the immunological properties of analytes.

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