| 書目名稱 | Enzyme Engineering |
| 副標題 | Methods and Protocol |
| 編輯 | James C. Samuelson |
| 視頻video | http://file.papertrans.cn/314/313063/313063.mp4 |
| 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts |
| 叢書名稱 | Methods in Molecular Biology |
| 圖書封面 |  |
| 描述 | .Whether the pursuit is commercially motivated or purely academic, engineering a novel biological catalyst is an enticing challenge. High-resolution protein structure analysis allows for rational alteration of enzyme function, yet many useful enzyme variants are the product of well-designed selection schemes or screening strategies. .Enzyme Engineering: Methods and Protocols. provides guidance to investigators wishing to create enzyme variants with desired properties. This detailed volume covers such topics as a simple method for generating site-specific mutations within bacterial chromosomes. It also highlights the engineering of two difference types of rare-cutting endonucleases that show great potential in gene therapy applications: The newest development is the emergence of TAL effector nucleases or TALENs. Chapters describe newly developed technologies in sufficient detail so that each method can be practiced in a standard molecular biology laboratory. Written in the successful .Methods in Molecular Biology?. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and |
| 出版日期 | Book 2013 |
| 關鍵詞 | bacterial chromosomes; biological catalyst; enzyme function; gene therapy; protein engineering |
| 版次 | 1 |
| doi | https://doi.org/10.1007/978-1-62703-293-3 |
| isbn_softcover | 978-1-4939-6287-7 |
| isbn_ebook | 978-1-62703-293-3Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
| issn_series | 1064-3745 |
| copyright | Springer Science+Business Media New York 2013 |
|Archiver|手機版|小黑屋|
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