Titlebook: Enzyme Engineering; Methods and Protocol Francesca Magnani,Chiara Marabelli,Francesca Parad Book 2022 The Editor(s) (if applicable) and The

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Marcella Niglio,Cosimo Damiano Vitalete entrance tunnels in order to fine-tune enzymatic activity, regulate substrate specificity, or control reaction promiscuity..In this chapter, we describe the use of a rational in silico design and screening method to engineer the access tunnel of a fructosyl peptide oxidase with the aim to facilit

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1064-3745 and timely, .Enzyme Engineering: Methods and Protocols. is an ideal guide for both the novice and the veteran researcher interested in biocatalysis..Chapter 13 is available open access under a CC BY 4.0 license..978-1-0716-1828-8978-1-0716-1826-4Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Recombination of Compatible Substitutions by 2GenReP and InSiRePic properties. However, the question of how to efficiently explore the protein sequence space when ten or more beneficial substitutions are identified has not yet been addressed. Two recombination strategies 2GenReP and InSiReP employing CompassR are systematically investigated to minimize experimen

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Resurrecting Enzymes by Ancestral Sequence Reconstructionisclosing the evolutionary history—.., the phylogeny—of a protein family of interest and then inferring the sequences of its ancestors—.., the nodes in the phylogeny. Assisted by gene synthesis, the selected ancestors can be resurrected in the lab and experimentally characterized. The crucial step t

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