Titlebook: Environmental Monitoring of Bacteria; Clive Edwards Book 1999 Humana Press 1999

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https://doi.org/10.1057/9780230288119niques are time consuming, labor-intensive, and give results that are only of retrospective value. For example, a typical pathogen test (e.g., .) can include pre-enrichment, selective enrichment, selective plating, and confirmation can take up to 7 d to complete.

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New Schools and Educated People,thout a culture step. A further problem of the study of naturally occurring bacteria is the numbers of cells involved. Bacterial populations consist of immense numbers, and finding specific cells against a background that may consist of billions of nontarget cells is time-consuming and difficult.

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Recovery and Analysis of Ribosomal RNA Sequences from the Environment,ips of the uncultured taxa to be determined (.,.). The universally distributed genes most commonly used for such analyses are the rRNA genes, particularly those encoding the small ribosomal subunit RNAs (16S and 18S rRNA). rRNA genes have many advantages over other candidate genes, including the following:

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Book 1999thods for the environmental monitoring of bacterial populations. These state-of-the-art methods include techniques for the recovery of cells, for the analysis of whole cells, and for the molecular study of target species and heterogeneous populations.Many of the methods enable bacteria to be monitor

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Book 1999research in recent years that has challenged some long-held principles of traditional microbiology, Environmental Monitoring of Bacteriagives timely and practical access to powerfultechniques that allow the study of bacterial ecology as communities, as single cells, or at the molecular level. . ....

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Some Problems Posed by Natural Environments for Monitoring Microorganisms,. Unfortunately, it is often the case that experimental protocols developed for model species are not transposable to other bacteria. This gulf is often most apparent when deductions made from organisms grown as pure cultures in the laboratory are assumed to apply to those occurring in natural envir

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