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Titlebook: Engineering of Halogenases towards Synthetic Applications; Increasing the Therm Hannah Minges Book 2017 Springer Fachmedien Wiesbaden GmbH

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發(fā)表于 2025-3-21 19:41:04 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
書(shū)目名稱Engineering of Halogenases towards Synthetic Applications
副標(biāo)題Increasing the Therm
編輯Hannah Minges
視頻videohttp://file.papertrans.cn/312/311035/311035.mp4
概述Evolution and investigations on halogenases.Includes supplementary material:
叢書(shū)名稱BestMasters
圖書(shū)封面Titlebook: Engineering of Halogenases towards Synthetic Applications; Increasing the Therm Hannah Minges Book 2017 Springer Fachmedien Wiesbaden GmbH
描述Hannah Minges focuses on the investigation of two different FAD-dependent halogenases in order to analyze and improve their applicability for chemoenzymatic approaches in chemistry. Owing to beneficial features, like high selectivity and benign reaction conditions, nature’s toolkit for halogenation provides several advantages, whereas conventional chemical strategies require hazardous reagents and suffer from low selectivity. Therefore, enzymatic halogenation arises as promising alternative in the synthesis of valuable chemicals. One project focuses on the generation of a thermostable variant of the tryptophan halogenase Thal by means of directed evolution. The second project deals with the investigation of the marine halogenase Bmp5. This enzyme is of synthetic interest because it preferably introduces bromine into phenol compounds, whereas chlorination cannot take place.?.
出版日期Book 2017
關(guān)鍵詞Thal; Bmp5; Directed Evolution; Enzyme; Biocatalysis
版次1
doihttps://doi.org/10.1007/978-3-658-18410-0
isbn_softcover978-3-658-18409-4
isbn_ebook978-3-658-18410-0Series ISSN 2625-3577 Series E-ISSN 2625-3615
issn_series 2625-3577
copyrightSpringer Fachmedien Wiesbaden GmbH 2017
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Material and Methods, proteins, but also amino acids, metabolites etc. can be examined. The principle of separation is based on the analyte’s different adsorption to the stationary phase and its interaction with the mobile phase. In normal phase HPLC, a polar stationary phase generally consisting of silica media and a n
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Directed evolution of Thal: Discussion,stant variant by means of directed evolution. Therefore, the first and essential step was the generation of mutant libraries by introducing random mutations into the gene .. Commonly employed techniques are e.g. epPCR, iterative saturation mutagenesis, DNA shuffling or the usage of specialized mutat
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Summary,emoenzymatic approaches in organic chemistry. In contrast, conventional strategies for halogenation require hazardous reagents, e. g. molecular halogens, and suffer from low selectivity. To circumvent this issue establishment of enzymatic halogenation arises as a promising alternative.
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Hannah MingesEvolution and investigations on halogenases.Includes supplementary material:
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The Geology of Stratigraphic Sequencesrease reaction rates to a million fold without being consumed or permanently altered by the reaction. An interesting and useful aspect to employ enzymes in organic chemistry is their chirality and unique stereostructure, resulting in a high substrate specificity as well as regio- and stereoselectivi
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