Titlebook: Engineering and Application of Pluripotent Stem Cells; Ulrich Martin,Robert Zweigerdt,Ina Gruh Book 2018 Springer International Publishing

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Scalable Cardiac Differentiation of Pluripotent Stem Cells Using Specific Growth Factors and Small pproaches will require efficient and highly reproducible processes for the mass production of well-characterized CM batches..On their way toward beating CMs, hPSCs initially undergo an epithelial-to-mesenchymal transition into a primitive-streak (PS)-like population that later gives rise to all endo

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Targeted Gene Editing in Human Pluripotent Stem Cells Using Site-Specific Nucleases,ative therapies and biomedical research. With the advancement of ZFNs, TALENs, and the CRISPR/Cas9 technology, straightforward and precise manipulation of the genome of human pluripotent stem cells (PSC) became possible, allowing relatively easy and fast generation of gene knockouts, integration of

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Acquired Genetic and Epigenetic Variation in Human Pluripotent Stem Cells, selective growth advantage in culture. Additionally, there is evidence that reprogramming of human induced pluripotent stem cells (hiPSCs) introduces mutations. This poses a challenge to both the safety of clinical applications and the reliability of basic research using hPSCs carrying genomic vari

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Requirements for Using iPSC-Based Cell Models for Assay Development in Drug Discovery,in vitro systems are being developed to overcome some of these challenges. In particular, induced pluripotent stem cells (iPSCs) have provided the opportunity to generate human cell types that can be utilized for developing more disease-relevant cellular assay models. As the use of these complex mod

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https://doi.org/10.1007/978-3-319-73591-7pluripotency; small molecules; Transgene-Free iPSCs; Genome engineering; drug screening; tissue engineeri