Titlebook: Engineering and Analyzing Multicellular Systems; Methods and Protocol Lianhong Sun,Wenying Shou Book 2014 Springer Science+Business Media N

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https://doi.org/10.1007/978-3-031-23054-7To resolve this, we have applied synthetic biology principles to engineer human commensal microbe that can specifically sense and kill an antibiotic-resistant strain of .. In this chapter, we describe the methods used to assemble, characterize, and evaluate the effectiveness of our engineered microb

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https://doi.org/10.1057/9781137483553undergo dynamic changes because of cell–cell and cell–environment interactions. Transcriptome analysis allows us to study the molecular basis of these changes in cell physiology. For transcriptome analysis, it is essential that the cell populations in the coculture are harvested separately. Here, we

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The Evolution of the British Welfare States. Biofilm conformation can be altered during typical sample preparation for FISH, which can impact observations in multispecies biofilms, including the relative positions of cells. Here, we describe methods to preserve 3-D structure during FISH for visualization of an anaerobic coculture biofilm of

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https://doi.org/10.1007/978-3-319-17476-1le detailed molecular characterization of living bacterial colonies directly from nutrient agar. The ability to detect molecular signatures of living microbial communities is important for investigating metabolic exchange between species without affecting the viability of the colonies. We describe t

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,De Pougy’s Innovative Courtesan Fiction,ntrolling natural systems. Concurrently, the rise in open-source software has greatly increased the ease at which researchers can implement their own mathematical models. With a reasonably sound understanding of mathematics and programming skills, a researcher can quickly and easily use such tools f