Titlebook: Engineered Zinc Finger Proteins; Methods and Protocol Joel P. Mackay,David J. Segal Book 2010 Springer Science + Business Media, LLC 2010 A

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Preparing Your Media in iTunes,ion molecule and against the promoter of the RNA component of telomerase. Methods to assess DNA binding of the engineered ZFP as well as to determine and improve the cellular effect of ATFs on (endogenous) promoter activity are described.

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Leitlinien zur Verwundetenversorgungin a sequence-specific manner. Here, basic methods that are helpful in adapting the engineered zinc finger technology for targeting the DNA in mitochondria are presented with the main emphasis on mitochondrial import of zinc finger proteins.

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https://doi.org/10.1007/978-1-60761-753-2Artificial transcription factors; DNA; DNA binding domains; Designer ZFPs; ZFP construction; Zinc finger

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Leitlinien zur Verwundetenversorgunge endogenous target. Here we describe a simple procedure for quantifying mutations that result from DNA double-strand break repair via non-homologous end joining. The assay is based on the ability of the Surveyor nuclease to selectively cleave distorted duplex DNA formed via cross-annealing of mutated and wild-type sequence.

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