Titlebook: Endocannabinoid Signaling; Methods and Protocol Mauro Maccarrone Book 2016 Springer Science+Business Media New York 2016 Cannabis sativa.en

agnostic

使人煩燥

Methods in Molecular Biologyhttp://image.papertrans.cn/e/image/309696.jpg

稱贊

https://doi.org/10.1007/978-3-540-34282-3s able to bind to a specific receptor with high affinity. Here, we describe the displacement binding assay that is carried out with a radioligand and CHO (Chinese Hamster Ovarian) cells stably transfected with the human cannabinoid CB. receptor.

單調(diào)女

商談

Hyperalgesia

NoC Reuse for SoC Modular Testing, the biological effects of this lipid mediator. The final products of this reaction are arachidonic acid and ethanolamine. In the method described herein, FAAH activity is measured through the use of a radioactive substrate by quantification of reaction products, that is, [.C]-ethanolamine from [.C-ethanolamine]-AEA.

Adenocarcinoma

The Displacement Binding Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells,s able to bind to a specific receptor with high affinity. Here, we describe the displacement binding assay that is carried out with a radioligand and CHO (Chinese Hamster Ovarian) cells stably transfected with the human cannabinoid CB. receptor.

模仿

The Cyclic AMP Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells,f G-protein-coupled receptor (GPCR) ligands. Here, we describe the cyclic AMP assay that is carried out with commercially available non-radioligand ready-to-use kits and Chinese hamster ovarian (CHO) cells stably transfected with the human cannabinoid CB. receptor.

拋媚眼

Assay of NAPE-PLD Activity,(FAAs), a family of bioactive lipids including anandamide (AEA) as the prototypical member. Here, we describe a NAPE-PLD assay based on radioactive substrates and product separation by thin-layer chromatography (TLC).

GNAT