Titlebook: Endocannabinoid Signaling; Methods and Protocol Mauro Maccarrone Book 2016 Springer Science+Business Media New York 2016 Cannabis sativa.en

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Assay of NAT Activity, chain is transferred from the .-1 position of the donor phospholipid, such as phosphatidylcholine, to the amino group of phosphatidylethanolamine, resulting in the formation of .-acylphosphatidylethanolamine. NAT has long been known to be stimulated by Ca., and hence it has been referred to as Ca.-

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Assay of Monoacylglycerol Lipase Activity,-AG is one of the main endogenous lipid agonists for cannabinoid receptors in the brain and elsewhere in the body. In the central nervous system (CNS), MGL is localized to presynaptic nerve terminals of both excitatory and inhibitory synapses, where it helps control the regulatory actions of 2-AG on

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A Sensitive and Versatile Fluorescent Activity Assay for ABHD12, enzymes in this large protein class has remained unclear. One recently found mSH is α/β-hydrolase domain containing 12 (ABHD12) enzyme, whose natural substrate in vivo appears to be the lysophospholipid lysophosphatidylserine (LPS). In vitro, ABHD12 together with monoacylglycerol lipase (MAGL) and

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