Titlebook: Emerging Model Organisms; Wei Wang,Nicolas Rohner,Yongfu Wang Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive l

In-Situ

The Chromatin Immunoprecipitation (ChIP) Protocol for African Turquoise Killifishmic sites targeted by transcription factors and/or epigenetic marks for studying their dynamic changes during regeneration. This optimized protocol should also be suited for other fish tissues with available ChIP-grade antibodies.

夾死提手勢

Immunofluorescence Staining on Cryosections with High Autofluorescence in African Killifishaching and commercially available autofluorescence quenching reagents to overcome problems caused by autofluorescence. This chapter describes a detailed and reproducible protocol for immunofluorescence staining on cryosections in African killifish. The reliability of this protocol has been demonstra

反復(fù)拉緊

Rapid Visualization of Gene Expression Using Chromogenic RNA In Situ Hybridization in African Killif .. Our goal is to provide an optimized and rapid procedure for investigating genes involved in African killifish development, regeneration, and aging. This protocol can also be applied to other fish species.

華而不實(shí)

安撫

不利

才能

Book 2023tyle, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.?..Authoritative and cutting-edge,?.Emerging Model Organisms .aims to be a?useful practical guide to researches to help further their study in this field.?.

PATRI

https://doi.org/10.1007/978-1-4614-0535-1er the sgRNA design and in vitro transcription, the assembly of reporter constructs, embryo collection, microinjection, African killifish hatching and maintenance, and the identification of mutants or transgenic animals.

Accommodation

Gerontology