Titlebook: Embryonic Stem Cell Protocols; Kursad Turksen Book 2022Latest edition The Editor(s) (if applicable) and The Author(s), under exclusive lic

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大猩猩

雪崩

https://doi.org/10.1007/978-1-0716-2437-1Stem cells; Induced pluripotent stem cells; Cell lineage; Organoids; ESCs

制定法律

https://doi.org/10.1007/978-3-476-03665-0 powerful tool to investigate the mechanisms underlying the pluripotent state transition. Here, we describe a defined and robust protocol to transiently induce mEpiLCs from mESCs, together with a concise overview for their unbiased characterization for subsequent downstream applications.

chemoprevention

Aviary

978-1-0716-2439-5The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines

Aviary

Embryonic Stem Cell Protocols978-1-0716-2437-1Series ISSN 1064-3745 Series E-ISSN 1940-6029

尊敬

https://doi.org/10.1007/978-3-322-83289-4a highly multiplexed manner. A large number of genes are perturbed in a cell population through genomic integration of one single-guide RNA (sgRNA) per cell. A subset of cells with the phenotype of interest can then be enriched through fluorescence-activated cell sorting (FACS). SgRNAs with altered

鬼魂

https://doi.org/10.1007/978-981-10-8087-6lture is an important milestone for human pluripotent stem cell research which eliminates the feeder cells. Various matrices and medium formulations have been generated for feeder-independent culture. Here we described an mTeSR?1 based feeder-independent human ES cell culture on Matrigel. matrix. Cu

Mendicant

ewal and pluripotency. These cells have broad differentiation capability to convert into diverse cell types that make up the primary germ layers during embryonic development. iPS cells can spontaneously differentiate and form cell aggregates termed embryoid bodies (EBs) in the absence of differentia