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Titlebook: Electroporation Protocols; Preclinical and Clin Shulin Li,Jeffry Cutrera,Justin Teissie Book 2014Latest edition Springer Science+Business M

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書目名稱Electroporation Protocols
副標(biāo)題Preclinical and Clin
編輯Shulin Li,Jeffry Cutrera,Justin Teissie
視頻videohttp://file.papertrans.cn/307/306527/306527.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Electroporation Protocols; Preclinical and Clin Shulin Li,Jeffry Cutrera,Justin Teissie Book 2014Latest edition Springer Science+Business M
描述.Electroporation gene therapy, or gene electrotransfer, has evolved greatly over the last few decades as a result of the remarkable progress in genetic sequencing, gene array analysis, gene cloning, gene expression detection, DNA manufacture and discovery and synthesis of siRNA. .Electroporation Protocols: Preclinical and Clinical Gene Medicine, Second Edition. provides in-depth knowledge on the delivery of naked DNA and small-interfering RNA (siRNA) to the targeted cells, tissues, and animals for prevention and treatment of disease. It builds on the success of the first edition and on the progress made in siRNA delivery and DNA vaccines for large animals as well as discovery of electroporation applications for the fragile tissues and for internal organs. Written in the successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls..Authoritative and easily accessible,. .Electroporation Protocols: Preclinical and Clinical Gene Medicine, Second Edition. aims to provide not only compre
出版日期Book 2014Latest edition
關(guān)鍵詞DNA transfer; DNA vaccines; SiRNA electrotransfer; electroporation therapy; gene delivery; gene expressio
版次2
doihttps://doi.org/10.1007/978-1-4614-9632-8
isbn_softcover978-1-4939-5492-6
isbn_ebook978-1-4614-9632-8Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2014
The information of publication is updating

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Substrate-Mediated, High-Efficiency siRNA Electroporation carrying siRNA. After the cells are adhered on the electrode surface, an electric pulse is applied to release siRNA from the surface and transfect it into the cells. A siRNA electroporation microarray is also presented for high-throughput screening of the functions of many siRNAs.
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Interkulturelles Marketing-Managementection efficiency and low cytotoxicity. However, owing to trade secrecy, the components in each buffer are unknown, which not only increases the cost of electroporation studies but also limits the application of Nucleofector in clinical cell-based gene therapies. Thus, we developed a three-step meth
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Der Bedarf an interkultureller Kompetenz, cell type. The commercial electroporation systems have been widely adopted in research and clinics with protocols usually compromising appropriate transfection efficiency and cell viability. By introducing gold nanoparticles (AuNPs), we demonstrated greatly enhanced performance of electroporation f
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