Titlebook: Electron Probe Microanalysis; Applications in Biol Karl Zierold,Herbert K. Hagler (Associate Professo Conference proceedings 1989 Springer-

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CONE

https://doi.org/10.1007/978-3-642-74477-8X-ray; cells; diagnosis; spectroscopy; tissue

Anticonvulsants

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征稅

https://doi.org/10.1007/978-3-476-05318-3he cellular ions are captured in a life-like state. Due to the fact that electron microscopic analysis of morphology and ion distribution of frozen-hydrated preparations still poses severe technical problems (Zierold, 1986) different techniques are commonly used to obtain stable dehydrated specimens

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Karl Zierold,Herbert K. Hagler (Associate Professo

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0932-2353 or physiologist. 3. Cells and tissues are not static but dynamic systems. Thus, for example, microanalysis of physiolo- gical processes requires sampling techn978-3-642-74479-2978-3-642-74477-8Series ISSN 0932-2353 Series E-ISSN 1868-2561

separate

Conference proceedings 1989ecimens or fully hydrated, frozen specimens. This data usually has to be con- verted into quantitative data meaningful to the cell biologist or physiologist. 3. Cells and tissues are not static but dynamic systems. Thus, for example, microanalysis of physiolo- gical processes requires sampling techn

Moderate

Freeze-Substitution and Low Temperature Embedding for Analytical Electron Microscopye Zierold and Steinbrecht, 1987) it is generally assumed that freeze-dried cryosections are best suited for microanalysis and that the retention of mobile ions at or near their original sites appears very unlikely after FS and embedding. Even if a considerable amount of mobile ions can be detected i