Titlebook: Electromanipulation in Hybridoma Technology; Carl A. K. Borrebaeck,Inger Hagen Book 1989 Palgrave Macmillan, a division of Macmillan Publi

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RADE

Electroporation of Human Lymphoid Cells,n human B-cell immortalization.. The techniques most widely employed to immortalize human B cells include classical hybridoma technology using a tumor cell fusion partner and cell transformation/immortalization accomplished by infecting the target B cells with Epstein-Barr virus (EBV).. Both methodo

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Generating Immortalized Immunoglobulin-secreting Human Lymphocytes by Recombinant DNA Technology,ells), with an appropriate fusion partner, typically a myeloma or lymphoblastoid cell line, via the cumbersome and somewhat unpredictable polyethylene glycol (PEG) procedure.. The fusion partner simply acts as a vector, supplying immortalization functions to an immunoglobulin-secreting lymphocyte. R

CHASE

B-Cell Hybridoma Production by Avidin-Biotin Mediated Electrofusion,ncements in the biomedical sciences and is widely used. The method is based on the random fusion of two cell types in the presence of polyethyleneglycol (PEG),. although viruses were used in earlier experiments.. Antibodies to a wide variety of antigens have been reported. Spleen cells from hyperimm

Figate

Hematological Disorders in Childrenved the way for the development of new tools in bioprocessing and genetic engineering.. Electroinjection (electropermeabilization or electroporation) of membrane-impermeable molecules of low and high molecular weight into living cells was the first application of this field pulse technique.. Researc

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Aakanksha Singh,Mrinalini Kotru Although it is likely that human monoclonal antibodies (HMAbs) will have distinct advantages, particularly in therapeutic applications, the ability to produce HMAbs has lagged far behind those developed for the murine system. Three general problems have limited the development of HMAbs: the lack of

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