Titlebook: Ebolaviruses; Methods and Protocol Thomas Hoenen,Allison Groseth Book 2017 Springer Science+Business Media LLC 2017 Ebola virus.Virology.Za

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Navin C. Nanda,Joel S. Raichlention of tagged and particularly fluorescent or luminescent viruses can be applied as tools for both diagnostic applications and for screening to identify novel countermeasures. Here we describe the generation and basic characterization of recombinant Ebola viruses rescued from cloned cDNA using a T7-driven system.

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項(xiàng)目

Forty Years of Ebolavirus Molecular Biology: Understanding a Novel Disease Agent Through the Developh, if not driven by, advancements in the technical methodologies available for these kinds of studies. Here we review of the current state of our knowledge regarding ebolavirus biology and emphasize the associated methods that made these discoveries possible.

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Nonradioactive Northern Blot Analysis to Detect Ebola Virus Minigenomic mRNArect study of .-acting regulatory regions as well as .-acting factors involved in Ebola virus minigenome transcription compared to the indirect measurement of reporter protein activity that additionally reflects translational effects (. Chapter . in this book for details).

宣誓書

Generation of Recombinant Ebola Viruses Using Reverse Geneticstion of tagged and particularly fluorescent or luminescent viruses can be applied as tools for both diagnostic applications and for screening to identify novel countermeasures. Here we describe the generation and basic characterization of recombinant Ebola viruses rescued from cloned cDNA using a T7-driven system.

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Luciferase-Expressing Ebolaviruses as Tools for Screening of Antivirals short assay times. Here we provide a detailed protocol for the use of such a luciferase-expressing reporter virus for antiviral screening in a 96-well format, with parallel assessment of cytotoxicity of the screened compounds.

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A Semi-automated High-Throughput Microtitration Assay for Filovirusesng a luminescent-based cell viability assay and an automated plate reader, has improved workflow efficiency, reduced operator burden and assay time, decreased assay variability, and increased data return.