Titlebook: Early, rapid and sensitive veterinary molecular diagnostics - real time PCR applications; Erika Pestana,Sandor Belak,Gerrit J. Viljoen Boo

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,Real-Time PCR – The Basic Principles,R analysis. Real Time PCR overcome this problem, because of its ability to measure the PCR amplicons at early states of the reaction as they are accumulate in a “Real Time Detection” mode thus measuring the amount of PCR product where the reaction is still in the exponential phase (QPCR).

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Specifications for PCR Machines,is conveyed expressly, by implication or by estoppel under any other patent claim, such as claims to apparatus, reagents, kits, or methods such as 5’ nuclease methods. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

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Traditional PCR,1980s, Mullis, who was working at Cetus Corporation, imagined a way to start and stop a polymerase’s action at specific points along a single strand of DNA. Cetus scientists eventually succeeded in artificially multiply DNA through repeated cycles of duplication driven by an enzyme called DNA polyme

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,Real-Time PCR – The Basic Principles,ementary to the sequence of interest. Primers are extended by the DNA polymerase. The copies produced after the extension, so called amplicons, are re-amplified with the same primers leading thus to an exponential amplification of the DNA molecules. After amplification, gel electrophoresis is used t

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New Trends in the Diagnosis and Molecular Epidemiology of Viral Diseases,ng (stamping out policies) and regulation of animal movements; various virus diseases still have a very high negative impact on animal health and welfare. The intensification of animal husbandry; centralisation of large groups of animals in industrial production units; globalization of trade in live

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Disease Diagnosis Using Real-Time PCR Specific Procedures for Important Veterinary Pathogens,h a variety of PCR systems in order to illustrate such developments. The evolution of methods involves many stages and applied analyses and inevitably different stages are reflected in publication of operating procedures or protocols. This is true in the following descriptions which, although called

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