Titlebook: E. coli Plasmid Vectors; Methods and Applicat Nicola Casali,Andrew Preston Book 20031st edition Springer Science+Business Media New York 20

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1064-3745 d cloning vehicles, such as cosmids, bacterial artificial chromosomes, l vectors, and phagemids. Common downstream applications such as mutagenesis of plasmids, recombinant protein expression, and the use of reporter genes, are also described.978-1-61737-391-6978-1-59259-409-2Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Transport in Single Channel Quantum Wires,ic manipulation has subsequently solidified the role of . as the host of choice for the propagation, manipulation, and characterization of recombinant DNA. In the past 60 yr . has been the subject of intensive research and more is now known about these bacilli than any other organisms on earth.

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3.524, Protocol 10). Plasmid minipreps are a ubiquitous part of molecular biology. They are simple and easy to perform but can be very tedious when large numbers of samples are involved. In recent years, endeavors such as the human genome project have led to the development of methods for the manageable high-throughput extraction of plasmids (.–.).

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High-Throughput Plasmid Extraction Using Microtiter Plates,, Protocol 10). Plasmid minipreps are a ubiquitous part of molecular biology. They are simple and easy to perform but can be very tedious when large numbers of samples are involved. In recent years, endeavors such as the human genome project have led to the development of methods for the manageable high-throughput extraction of plasmids (.–.).

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Cubic Silicon Carbide Nanowires,: insert size, copy number, incompatibility, selectable marker, cloning sites, and specialized vector functions. Several of these criteria are dependent on each other. This chapter discusses these criteria in the context of choosing a plasmid for use as a cloning vector and . displays the features of some commonly used cloning vectors.

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