Titlebook: E. coli Plasmid Vectors; Methods and Applicat Nicola Casali,Andrew Preston Book 20031st edition Springer Science+Business Media New York 20

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Extraction of DNA from Agarose Gels, elution of the DNA from the substrate. Other methods include hot phenol extraction of the DNA from the gel. The use of phenol is best avoided if possible because of safety and waste disposal concerns. Presented here are two alternative techniques that avoid these concerns, do not require the purcha

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,Construction of Genomic Libraries in λ-Vectors,cloning sites facilitating the cloning of foreign DNA. In addition, λ-phage vectors have the following advantageous features: high cloning efficiency, a relatively large insert-size capacity, and suitability for screening using nucleic acid probes. In terms of gene screening, genomic DNA libraries a

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Isolation of Plasmids from , by Boiling Lysis,described with different adaptations in a variety of protocol books (.,.). The quality of the isolated plasmid DNA is lower than that from an alkaline lysis miniprep, but it is sufficient for restriction analysis.

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Cloning in Plasmid Vectors,d upon transformation of the recombinant molecule into a bacterial cell (.. and .) so that the DNA of interest can be investigated further. Cloning is an essential part of many experiments, including library generation (..) and expression studies (..).

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E. coli Plasmid Vectors978-1-59259-409-2Series ISSN 1064-3745 Series E-ISSN 1940-6029

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