Titlebook: Dynein; Methods and Protocol Steven M. Markus Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive license to Springe

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Live-Cell Imaging of Dynein-Mediated Cargo Transport in rganism used for studying dynein function and regulation in vivo. Here, we describe how we grow . strains for live-cell imaging and how we observe the dynein-mediated distribution of early endosomes and secretory vesicles. Using an on-stage incubator and culture chambers for inverted microscopes, we

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Employing Live-Cell Imaging to Study Motor-Mediated Transport including activating adaptors and scaffolding proteins, and microtubule tracks that also provide regulatory cues. While in vitro studies are invaluable, fully replicating the physiological conditions under which motility occurs in cells is not yet possible. Here, we describe two methods that can be

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Assessment of Dynein-Mediated Nuclear Migration in the Developing Cortex by Live-Tissue Microscopy termed interkinetic nuclear migration. The nucleus of postmitotic neurons derived from these neural stem cells also translocates in a saltatory manner to enable neuronal migration toward the cortical plate. In these processes, various molecular motors, including cytoplasmic dynein, myosin II, and k