Titlebook: Dynein; Methods and Protocol Steven M. Markus Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive license to Springe

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Wissenschaftliche Reisen mit Loki Schmidtt the single-molecule level have not been studied widely. Here, we describe how highly inclined and laminated optical sheet (HILO) microscopy can be adapted to visualize single molecules of the motor protein cytoplasmic dynein-1 in mammalian cells with high signal-to-noise ratio and temporal resolution.

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High-Resolution Tracking of Dynein-Dynactin-BicD2 Complexesined by tracking gold nanoparticle-labeled DDB complexes with interferometric scattering (iSCAT) microscopy and using an algorithm to separate out different motility phases. Here we describe methods for purifying DDB complexes from brain lysate, labeling with gold nanoparticles, imaging by iSCAT, and analyzing the resulting trajectories.

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Problemstellung und Zielsetzung, can image fungal hyphae that naturally attach to the bottom of the chambers, using wide-field epifluorescence microscopes or the new Zeiss LSM 980 (with Airyscan 2) microscope. In addition to methods for preparing cells for imaging, a procedure for . transformation is also described.

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Zur Beatmung der verschiedenen Wehen,tion and manipulate downstream events. In this chapter, I will present methods for locally activating cytoplasmic dynein at the mitotic cell cortex in human cells, with a focus on how to generate knock-in cell lines and set up a microscope system.