Titlebook: Downstream Processing of Proteins; Methods and Protocol Mohamed A. Desai Book 2000 Humana Press 2000

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Downstream Processing in the Biotechnology Industry,thods of medicine. The pain-killing salicylates and antimalarial compounds extracted from the bark of certain trees are notable examples of older medicines. Similarly, animal organs such as the pancreas, placenta, and the urine of pregnant females have been a source of hormones for therapeutic use.

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3 Protein Concentration and Buffer Exchange Using Ultrafiltration,nd desalting, (3) removal of small contaminants, (4) protein purification, and (5) virus clearance. This chapter will consider the first three applications-other chapters in this volume discuss the final two processes. Separation in UF is primarily owing to differences in solute size, with the large

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4 High-Performance Tangential Flow Filtration for Protein Separations, limit to their relative size (.). HPTFF can be used throughout the purification process to remove specific impurities (e.g., proteins, DNA, or endotoxins), clear viruses, and/or eliminate protein oligomers or degradation products. In addition, HPTFF can effect simultaneous purification, concentrati

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Application of Density Gradient Ultracentrifugation Using Zonal Rotors in the Large-Scale Purificatd mac-romolecules in a range of processes. Its application has been of particular significance in the commercial preparation of viruses for vaccine and immuno-therapy products in both batch and continuous-flow zonal modes. These methods have been traditionally used to purify influenza vaccines (.),

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