Titlebook: Directed Evolution Library Creation; Methods and Protocol Frances H. Arnold,George Georgiou Book 2003 Humana Press 2003

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Gaben von Nachiketas und Savitriutagenesis has been performed using DNA-modifying chemicals or UV radiation. An alternative is the use of so-called mutator strains, which carry defects in one or several DNA repair genes. Such mutator strains have been used for the directed evolution of individual genes on plasmids or phagemids (.,

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Postmodernismus und Sri Aurobindo). Several methods for introducing random mutations in vitro have been reported (.). Among these, error-prone PCR mutagenesis, based on inaccurate copying by DNA polymerase, is the most commonly used technique to introduce random point mutations (.). However, the error-prone PCR method has an inhere

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bizarre

https://doi.org/10.1007/978-981-97-3027-8proteins (library) for functional improvements. By targeting mutations to certain amino acids, we can map the enzyme active site, investigate mechanisms, and study structure-function relationships. With saturation mutagenesis, it is possible to create a library of mutants containing all possible mut

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