Titlebook: Directed Evolution Library Creation; Methods and Protocol Elizabeth M.J. Gillam,Janine N. Copp,David Ackerle Book 2014Latest edition Spring

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Transposon-Based Approaches for Generating Novel Molecular Diversity During Directed Evolutionon endonuclease generates blunt-end random breaks at a frequency of one per target gene and the concomitant loss of 3 bp. Self-ligation or insertion of another DNA cassette results in the sampling of trinucleotide deletion or trinucleotide substitution/domain insertion, respectively.