Titlebook: Diffusion Chamber Culture; Hemopoiesis, Cloning Eugene P. Cronkite,Arland L. Carsten Conference proceedings 1980 Springer-Verlag Berlin Hei

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Megakaryocyte Growth in In Vitro Cultures and in Diffusion Chambersmean number of endoreduplications per colony megakaryocyte and the number of doublings undergone by the colony progenitor. Megakaryocytes can also be grown in diffusion chambers although with a lower efficiency and smaller colony size than in plasma clot cultures.

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c attempts to use this methodology to study cell proliferation (review by Carsten, Chap. 1). Not so long ago diffusion chamber culture was nicknamed "confusion chamber" culture. I believe this conference has removed the confusion and will truly point out the intrinsic value of the system. It is not

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CO2 Emissions by Germany and the UK and human tumour cells taken directly from patients. This technique has been used to study the sensitivity of the clonogenic cells to a variety of cytotoxic agents and to compare the sensitivities of human marrow and tumour cells. A wide range of differential chemosensitivity is observed.

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History and Overview of the In Vivo Diffusion Chamber (DC) Culture Systemontainer to the modern systems consisting of single- and multichambers with differing wall materials. Various applications of the system and methods of analysis are discussed. The many advantages and disadvantages of this system are considered as compared with other available culture systems.

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Roles of CFU-S and CFU-C in Maintaining Cell Growth in Diffusion Chamber Cultures of Murine Marrowapid growth and differentiation of granulocytic cells followed by a prolonged steady-state of granulocyte renewal. In studies to assess the roles of CFU-S and CFU-C in the initiation and maintenance of granulocyte growth in this model of normal granulopoiesis, we have found rapid fluctuations in the

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Human Granulocytopoietic Progenitor Cell Diversity and Regulation Studied in Diffusion Chambers CFU-D which form neutrophilic colonies in diffusion chambers in irradiated mice can be separated from day 14 CFU-C and day 7 CFU-C which form colonies in agar cultures in vitro in response to leukocyte-derived colony stimulating activity (CSA). Kinetic studies suggest that these cells are serially

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Studies on the Regulation of Diffusion Chamber Granulopoiesisth DC with 0.5 x 10. normal marrow cells and then injected for 3–4 days intravenously with 5 .g . endotoxin or saline. There were no significant increases in DC myelopoiesis, but significant increases in host animal total tibial proliferative granulocytes on day 4 and suggestive increases in nonprol

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Megakaryocyte Growth in In Vitro Cultures and in Diffusion Chambers are programmed to) undergo a very variable number of proliferative cycles before switching to polyploidization. Alternatively the size and ploidy distributions reflect an age structure in the progenitor population. In the in vitro plasma culture system, there is an inverse relationship between the