Titlebook: Differential Display Methods and Protocols; Peng Liang,Jonathan D. Meade,Arthur B. Pardee Book 2006Latest edition Humana Press 2006 DNA.Mi

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Vincenzo Parenti-Castelli,Werner Schiehlenhue of anthocyanin molecules. However, anthocyanin 5-.-glucosyltransferase (5-GT) is unstable and it had been difficult to purify this enzyme by biochemical methods. The isolation of cDNA encoding 5-GT was succeeded first by mRNA differential display (DD) of red and green chemotypes of . var. .. The

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https://doi.org/10.1007/978-3-319-33714-2 genes bound and regulated by Xbp1, have been identified using differential display (DD). Because little was known about the regulation or function of Xbp1, we identified genes that were underrepresented in RNAs purified from cells in which Xbp1 was ectopically expressed. Four target genes, includin

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Manifest

Global Analysis of Gene Expression by Differential Displayan accurate guidance on how many DD polymerase chain reaction (PCR) primer combinations are needed to display most of the genes expressed in a eukaryotic cell. This study critically evaluated the gene coverage by DD as a function of the number of arbitrary primers, the number of 3′ bases of an arbit

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Ordered Differential Displayprovides means to browse through essentially all the transcripts in the compared mRNA pools. It is specifically adapted for small amounts of starting material. The protocol outlined here, in addition to ODD procedure itself, also describes isolation of RNA and synthesis of double-stranded cDNA from

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High-Density Sampling Differential Display of Prokaryotic mRNAs With RAP-PCR(RT-PCR) are performed on total RNA isolated from induced and control bacterial cultures. Each RT-PCR reaction uses a single oligonucleotide primer and constitutes an independent sampling of the mRNA population. The large number of reactions performed allows the repeated sampling of the targeted pol

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