Titlebook: Diagnostic Flow Cytometry in Cytology; Pranab Dey Book 2021 The Editor(s) (if applicable) and The Author(s), under exclusive license to Sp

Glower

https://doi.org/10.1007/978-1-349-11612-6The present chapter describes the techniques of sample preparation in flow cytometry procedure. The preparation of single cells and the staining of the cells by the appropriate fluorochrome dye are two critical steps of sample preparation. The chapter also discusses the machine’s setup, control sample, and data acquisition in flow cytometry.

Apoptosis

https://doi.org/10.1007/978-1-349-11612-6The present chapter discusses the display and interpretation of data in flow cytometry. The data at first is recorded in “l(fā)ist mode”. Further, the data is displayed as various graphs. The different types of histograms are used to display data. Selective gating of the cell population is often used to identify the subset population.

minion

比喻好

https://doi.org/10.1007/978-3-658-43864-7The present chapter discusses the various dye for the staining of nucleic acid in flow cytometry. The DNA-binding dye should be specific for DNA and should bind stoichiometric to the DNA content. With the help of DNA flow cytometry one can assess the ploidy and cell proliferative fraction of tumour.

古代

作嘔

https://doi.org/10.1007/978-94-009-8866-8In the present chapter, the various markers in flow cytometry are discussed. The predominant expression of these markers in lymphoma has been highlighted.

dithiolethione

Hegel—From Foundation to SystemThis chapter discusses the characteristic cytomorphology and flow cytometry (FCM) findings of B- non-Hodgkin lymphoma (NHL). Flow cytometry is more diagnostic in small and medium-sized lymphoma. Both cytomorphology and FCM findings are needed for the exact subtyping of B-NHL.

Lumbar-Spine

heckle

1分開

Sample Preparation and Data Acquisition in Flow CytometryThe present chapter describes the techniques of sample preparation in flow cytometry procedure. The preparation of single cells and the staining of the cells by the appropriate fluorochrome dye are two critical steps of sample preparation. The chapter also discusses the machine’s setup, control sample, and data acquisition in flow cytometry.