Titlebook: Diagnostic Bacteriology Protocols; Jenny Howard,David M. Whitcombe Book 19951st edition Humana Press 1995

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Conrado C. Gon?alves,Carlos H. I. RamosThe technique of pulsed field gel electrophoresis (PFGE) enables researchers to separate linear DNA fragments of up to 10 Mb. The technique was first described by Schwartz and Cantor (.) and differs from conventional gel electrophoresis by requiring the DNA molecules periodically to change their direction of migration.

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Conrado C. Gon?alves,Carlos H. I. RamosTotal DNA restriction pattern analysis is one of the techniques commonly used to identify and group bacteria (.–.). This technique can be used to separate species belonging to the same genus (.,.) or to distinguish highly similar bacterial strains within the same species or subspecies (.–.).

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The Direct Immunofluorescent Filter Technique (DIFT),Many areas of analytical microbiology that deal with biological materials (e.g., medicine and the food, water, and pharmaceutical industries) need methods of detecting and identifying microorganisms that can give results in a few hours rather than the many days required for conventional methods.

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Fortify

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Antibiotic Resistance Profiling,a bacterium is tested for resistance, it should be compared, quantitatively with a standard. This standard may be the resistance of a wild-type strain, a typical strain, or a “type” strain, appropriate to the test strain.

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Pyrolysis Mass Spectrometry,tra represent transient bacterial “fingerprints” that can be compared mathematically for relatedness. The method has potential for identification, classification, and typing of bacteria with a high degree of resolution and speed.