Titlebook: Diagnosis of Sexually Transmitted Diseases; Methods and Protocol Colin R. MacKenzie,Birgit Henrich Book 2012 Springer Science+Business Medi

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Guidelines for High-Resolution Genotyping of , Using Multilocus Sequence Analysis, typing (MLST) system able to differentiate closely related clinical strains, which makes it ideal for short-term epidemiology and outbreak investigations. It is based on five highly variable but stable target regions which are PCR amplified and DNA sequenced.

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Protocol for the Detection and Genotyping of Human Papillomaviruses Using a Liquid Bead Microarray al cancer control. Here, we describe a Luminex assay-based liquid bead microarray assay for genotyping 37 HPV types, which is objective, scalable, amenable to a high-throughput configuration, and has the potential to be automated.

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Protocol for the Use of Self-Reporting Duplex Mutation Primers to Detect PCR Products in the Diagnople, in which signal is generated by melting a duplex mutation primer during renaturation. The duplex mutation primers are much more specific than double-stranded DNA dyes like SYBR Green I and, unlike other probes, do not require the double-labeled synthesis of fluorophore and quencher on the same molecule.

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