Titlebook: Diabetic Nephropathy; Methods and Protocol Luigi Gnudi,David A. Long Book 2020 Springer Science+Business Media, LLC, part of Springer Natur

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Isolating Urinary Extracellular Vesicles as Biomarkers for Diabetic Diseaseeflect the physiological state of their cells of origin. Recently, urinary extracellular vesicles have emerged as a valuable source of biomarkers for kidney and systemic disease..Unfortunately, all existing methods for extracellular vesicle isolation from urine are time consuming and/or expensive. T

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Detlef Pollack,Volkhard Krech,Markus Heroructural information than traditional sectioning and staining alone. Given the addition of paraffin embedding to our method, this hybrid protocol offers a powerful approach to combine confocal or two-photon findings with histological and further immunofluorescent analysis within the same tissue.

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Soziologische Religionstheorie nach dem ,uorescent agent that can be used as a renal marker as it is filtrated and completely excreted through the kidneys into the urine without reabsorption or secretion and without accumulation in the skin. The data recorded in the device are then analyzed with “GFRmeasure,” an open-source, freely downloadable, and user-friendly software.

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Maike R?nnau-B?se,Klaus Fr?hlich-Gildhoffe, we detail an in vitro method to analyze glucose uptake in conditionally immortalized human podocytes (Saleem, J Am Soc Nephrol 13:630–638, 2002; Coward, Diabetes 54:3095–3102, 2005); this assay is useful for detecting changes in podocyte metabolism, nutrient sensing, and insulin sensitivity.

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https://doi.org/10.1007/978-3-658-16253-5s devised a set of new, quick, simple, and inexpensive techniques, based on hydrostatic filtration dialysis (HFD) of urine extremely suitable for diagnostic purposes. This novel approach represents a great potential for new diagnostics and understanding disease biology in general and brings the biomarker detection to the scope of all laboratories.

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