Titlebook: Diabetes Mellitus; Sabire ?zcan Book 2003 Humana Press 2003 diabetes

conformity

Fractionation Analysis of the Subcellular Distribution of GLUT-4 in 3T3-L1 Adipocytestranslocation because complete inhibition of GLUT-4 endocytosis only modestly increases plasma membrane-associated GLUT-4 protein without affecting the extent of insulin-stimulated GLUT-4 translocation (.–.). In contrast to GLUT-4, GLUT-1 is an intracellular and plasma membrane localized in the basa

LATHE

Visualization and Quantitation of Integral Membrane Proteins Using a Plasma Membrane Sheet Assayrelies on sonic disruption of cells that are coated with a positively charged molecule, which allows the exofacial surface of the PM to be drawn into contact with the solid support on which the cells were cultured. Sonication of these cells results in formation of a “l(fā)awn” of adherent membrane fragm

extemporaneous

Assaying AKT/Protein Kinase B Activitytissue extracts by quantifying its ability to catalyze phosphate incorporation into an exogenous substrate (outlined in .). This chapter illustrates techniques for immunoprecipitating Akt/PKB, choosing an appropriate substrate for the reaction, and optimizing the assay conditions.

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指耕作

Christoph Schwindt,Jürgen Zimmermann. Islets are comprised of a heterogeneous population of endocrine cells, including insulin-producing β-cells (approx. 65–70%), glucagon-secreting α-cells (20–25%), somatostatin-secreting δ-cells, and polypeptide (PP)-secreting cells. Much of the cellular and biochemical information concerning the me

Cursory

Tdd526

Affiliation

Lower Bounds and Exact Solution Approaches.), β-cells in culture (.), tissue samples (.), and cells transgenically expressing the insulin gene (.). Like all immunocytochemistry techniques, much depends on the availability of a high-quality primary antibody. Species-specific insulin antibodies are now available from a number of commercial so

尖牙

Duquesne Collective Action Network mRNA levels in both β-cell lines and purified islets has been performed by Northern blot analysis, using the full-length insulin cDNA as a labeled probe (.). This technique is described here, and is still the most useful method for detection of endogenous insulin mRNA levels (densitometry being app

extemporaneous

Terry Lamb,Aniko Hatoss,Shirley O’Neillapproaches has become more important in light of the emerging notion that the natural chromatin context affects the outcome of transcriptional activation in vivo (.). Chromatin can exert a regulatory effect on transcription by modulating the access of activators to DNA (.). Different posttranslation