Titlebook: Developmental Biology of the Sea Urchin and Other Marine Invertebrates; Methods and Protocol David J. Carroll,Stephen A. Stricker Book 2021

廢墟

https://doi.org/10.1007/978-3-662-68670-6rating tissues can elucidate potential effectors during this process. Here we describe an effective transfection protocol that allows the introduction of Dicer-substrate interference RNAs (DsiRNAs) for the modulation of gene expression and its characterization during regeneration.

贊美者

https://doi.org/10.1007/978-1-0716-0974-3Sea stars; Embryos; Evolution; Novel model systems; Regenerative medicine; Oocytes

ARC

JADED

周年紀(jì)念日

ASSAY

鍍金

Marine Nemertean Worms for Immunoblotting Studies of Oocyte Aging,ir ability to undergo normal fertilization. This chapter updates a previously published protocol for conducting immunoblotting analyses of oocyte maturation in marine nemertean worms by adding general methods for obtaining adult worms and for handling their gametes in experiments assessing oocyte aging.

古文字學(xué)

Reservation

Gene Editing in the Ascidian , and Tail Nerve Cord Formation,in just a few organisms, these knockout techniques are now widely applied. Here we describe simple techniques for applying the CRISPR-Cas9 system to study the development of the nerve cord in the ascidian ..

fatty-acids